Serosurveillance cuam tshuam nrog kev kwv yees qhov ntau ntawm cov tshuaj tiv thaiv kab mob hauv cov pej xeem tawm tsam ib qho kab mob.Nws pab ntsuas kev tiv thaiv kab mob ntawm cov pej xeem tom qab kis kab mob lossis txhaj tshuaj tiv thaiv thiab muaj kev siv hluav taws xob sib kis hauv kev ntsuas kev pheej hmoo kis tus kab mob thiab cov pej xeem tiv thaiv qib.Nyob rau tam sim no tus kab mob coronavirus 2019 (COVID-19) kis thoob qhov txhia chaw, serosurvey tau ua lub luag haujlwm tseem ceeb hauv kev ntsuam xyuas qhov tseeb ntawm qhov mob hnyav ua pa nyuaj ua rau tus mob coronavirus 2 (SARS-CoV-2) kis kab mob hauv ntau haiv neeg.Nws kuj tseem tau pab tsim cov ntsuas kev sib kis, xws li tus kab mob tuag taus (IFR).
Thaum kawg ntawm 2020, 400 serosurveys tau luam tawm.Cov kev tshawb fawb no tau ua raws li ntau hom immunoassays uas tau tsim los tshuaj xyuas cov tshuaj tiv thaiv kab mob SARS-CoV-2, feem ntau tsom rau tag nrho lossis ib feem ntawm cov pob txha (S) thiab nucleocapsid (N) cov proteins ntawm SARS-CoV-2.Hauv qhov xwm txheej tam sim no COVID-19 kis thoob qhov txhia chaw, cov kab mob sib kis tau tshwm sim nyob rau ntau thaj chaw hauv ntiaj teb, ua rau muaj kev sib txawv ntawm cov pej xeem ntawm lub sijhawm.Qhov tshwm sim no tau tawm tsam SARS-CoV-2 serosurveillance vim muaj kev tiv thaiv kab mob ntau ntxiv.
Cov kws tshawb fawb tau pom tias cov tshuaj tiv thaiv-SARS-CoV-2 cov tshuaj tiv thaiv kab mob muaj qhov ua kom lwj tom qab lub sijhawm convalescent.Cov xwm txheej zoo li no ua rau muaj feem cuam tshuam tsis zoo los ntawm immunoassays.Cov kev tsis zoo no tuaj yeem ua rau muaj kev cuam tshuam loj ntawm tus kab mob tiag tiag tshwj tsis yog lawv tau lees paub thiab kho sai sai.Tsis tas li ntawd, cov tshuaj tiv thaiv kab mob tom qab kis kab mob tshwm sim sib txawv raws li qhov hnyav ntawm kev kis tus kab mob - qhov mob hnyav dua COVID-19 feem ntau ua rau muaj qhov nce ntxiv ntawm cov tshuaj tiv thaiv kab mob sib piv rau cov kab mob me lossis asymptomatic.
Ntau qhov kev tshawb fawb tau pom cov tshuaj tiv thaiv kab mob kinetics rau rau lub hlis tom qab kis kab mob.Cov kev tshawb fawb no pom tau tias feem coob ntawm cov tib neeg hauv cov zej zog uas kis tus kab mob SARS-CoV-2 pom tias muaj mob me me lossis asymptomatic.Cov kws tshawb fawb ntseeg tias nws yog qhov tseem ceeb los ntsuas qhov kev hloov pauv ntawm cov tshuaj tiv thaiv kab mob, siv cov tshuaj tiv thaiv kab mob, thoob plaws qhov dav ntawm qhov mob hnyav.Hnub nyoog kuj tau suav tias yog ib qho tseem ceeb hauv cov kev tshawb fawb no.
Hauv kev tshawb fawb tsis ntev los no, cov kws tshawb fawb tau txheeb xyuas cov tshuaj tiv thaiv kab mob SARS-CoV-2 txog li 9 lub hlis tom qab kis kab mob, thiab luam tawm lawv cov kev tshawb pom hauvmedRxiv* preprint server.Nyob rau hauv txoj kev tshawb no tam sim no, ib pawg ntawm cov tib neeg seropositive tau nrhiav los ntawm serosurveys ua hauv Geneva, Switzerland.Cov kws tshawb fawb tau siv peb yam tshuaj tiv thaiv kab mob sib txawv, uas yog, semiquantitative anti-S1 ELISA kuaj pom IgG (hu ua EI), qhov ntau ntawm Eecsys anti-RBD (hu ua, Roche-S) thiab semiquantitative Eecsys anti-N (hu ua Roche- N).Cov kev tshawb fawb tam sim no muab qhov kev nkag siab tseem ceeb rau cov pej xeem-raws li kev tshawb fawb serologic thiab qhia qhov nyuaj hauv thaj chaw tiv thaiv kab mob vim muaj kev sib xyaw ntawm cov kab mob COVID-19 tsis ntev los no thiab qhov nruab nrab, nrog rau kev txhaj tshuaj.
Txoj kev tshawb fawb raws li kev txiav txim siab tau tshaj tawm tias cov tib neeg uas tau cog lus rau COVID-19 nrog cov tsos mob me me lossis tsis muaj tsos mob, qhia pom muaj cov tshuaj tiv thaiv.Cov tshuaj tiv thaiv kab mob no tau tsom mus rau nucleocapsid (N) lossis cov kab mob sib kis (S) cov proteins ntawm SARS-CoV-2 thiab tau pom tias muaj kev pheej hmoo tsawg kawg 8 lub hlis tom qab kis kab mob.Txawm li cas los xij, lawv qhov kev kuaj pom yog nyob ntawm qhov kev xaiv ntawm immunoassay.Cov kws tshawb fawb tau pom tias kev ntsuas thawj zaug ntawm cov tshuaj tiv thaiv kab mob, coj los ntawm cov neeg koom hauv plaub thiab ib nrab lub hlis ntawm COVID-19, tau zoo ib yam thoob plaws peb hom tshuaj tiv thaiv kab mob siv hauv txoj kev tshawb no.Txawm li cas los xij, tom qab thawj plaub lub hlis, thiab txog li yim lub hlis tom qab kis kab mob, cov txiaj ntsig tau sib txawv ntawm qhov kev ntsuam xyuas.
Qhov kev tshawb fawb no tau qhia tias nyob rau hauv cov ntaub ntawv ntawm EI IgG kev soj ntsuam, ib ntawm plaub tus neeg koom tau sero-reverted.Txawm li cas los xij, rau lwm cov tshuaj tiv thaiv kab mob, xws li Roche anti-N thiab anti-RBD tag nrho cov kev ntsuam xyuas Ig, tsuas yog ob peb lossis tsis muaj sero-reversions raug kuaj pom rau tib tus qauv.Txawm tias cov neeg koom nrog cov kab mob me me, uas yav dhau los xav tias yuav ua rau cov lus teb tsis muaj zog tiv thaiv kab mob, tau pom tias muaj kev nkag siab zoo thaum siv cov tshuaj tiv thaiv RBD thiab anti-N tag nrho cov kev sim Ig Roche.Ob qhov kev ntsuam xyuas tseem ceeb rau ntau tshaj 8 lub hlis tom qab kis kab mob.Yog li, cov txiaj ntsig no tau qhia tias ob qho tib si Roche immunoassays yog qhov haum rau kwv yees seroprevalence tom qab lub sijhawm ntev tom qab pib kis tus kab mob.
Tom qab ntawd, siv cov kev ntsuas simulation, cov kws tshawb fawb tau txiav txim siab tias yam tsis muaj qhov ntsuas qhov tseeb, tshwj xeeb tshaj yog, txiav txim siab lub sijhawm sib txawv ntawm qhov kev ntsuas, qhov kev tshawb fawb seroprevalence yuav tsis raug.Qhov no yuav ua rau muaj kev kwv yees tsis txaus ntseeg ntawm tus lej tiag tiag ntawm cov kab mob sib kis hauv cov pej xeem.Txoj kev tshawb fawb immunoassay no tau pom tias muaj qhov sib txawv ntawm qhov sib txawv ntawm cov seropositivity ntawm cov khoom lag luam-muaj kev sim.
Nws yuav tsum raug sau tseg tias muaj ntau qhov kev txwv ntawm txoj kev tshawb no.Piv txwv li, cov reagent siv thaum ua qhov kev ntsuam xyuas EI rau ob qho tib si hauv paus (kev sim thawj zaug lossis thib 1) thiab kev soj ntsuam (kev sim thib 2 rau tib cov neeg sib tw) cov qauv hauv ib lub sijhawm tshwj xeeb sib txawv.Lwm qhov kev txwv ntawm txoj kev tshawb no yog tias cov pab pawg tsis suav nrog cov menyuam yaus.Txog niaj hnub no, tsis muaj pov thawj ntawm cov tshuaj tiv thaiv kab mob ntev ntev hauv cov menyuam yaus tau raug sau tseg.
Post lub sij hawm: Mar-24-2021